Characteristics of Penicillinase Release by Washed Cells of Bacillus licheniformis
نویسندگان
چکیده
منابع مشابه
Characteristics of penicillinase release by washed cells of Bacillus licheniformis.
Saline-washed cells of Bacillus licheniformis strain 749/C (constitutive for penicillinase) were able to release exopenicillinase in the presence of concentrations of chloramphenicol that prevented protein synthesis completely. The release reaction was strongly pH-dependent, occurring at a faster rate at alkaline pH in anionic or cationic buffers than at neutral pH. A strongly pH-dependent rele...
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Although the microbial formation of a variety of polypeptides possessing antibiotic activities has been known for some time, the biosynthesis of these compounds has as yet received little attention. Not only is the mechatnism of formation of these biologically active compounds of interest in itself, but the biosyntheses of these polypeptides are potential systems for investigating the general p...
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The penicillinase repressor (PENI) negatively regulates expression of the penicillinase gene (penP) in Bacillus licheniformis by binding to its operators located within the promoter region of penP.penI codes for a protein with 128 amino acids. Filter-binding analyses suggest that the active form of the repressor is a dimer. Genetic analyses of PENI derivatives showed that the repressor carrying...
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Penicillinase was localized in log-phase cells of Bacillus licheniformis 749/C by labeling with ferritin-anti-penicillinase immunoglobulin G conjugate. Mildly fixed homogenized cells, isolated subcellular fractions, and frozen thin sections were labeled. The label was distributed in discrete patches in the cell envelope. The patches extended from the inside part of the membrane to the outside p...
متن کاملPurification and characteristics of plasma membrane penicillinase from Bacillus licheniformis 749-C.
The plasma membrane-bound penicillinase of Bacillus licheniformis 749/C has been purified from bacteria grown in a medium containing [2-3H]glycerol and W-labeled aminoacids, and the purified enzyme compared with exopenicillinase. The procedure consisted of repeated chromatography on DEAE-Sephadex in the presence of Triton X-100, and gel filtration on Sephadex G-75 in the presence of taurodeoxyc...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1968
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.96.4.1231-1239.1968